ARS Heath Family Project Grant
The successful applicant for the ARS Heath Family Project Grant was:
Principal Applicant
Professor Helen Galley
Chair of Anaesthesia & Intensive Care, University of Aberdeen
Title
The effect of mitochondria targeted antioxidants on TRPV1 expression on dorsal root ganglion cells in paclitaxel induced neuropathy in vitro and in vivo.
Amount
£14,675
Scientific Abstract
Main research question
Do the chemotherapy drugs paclitaxel, cisplatin and oxaliplatin cause altered TRPV1 or TRPA1 channel expression on dorsal root ganglion cells (DRG), and can this be treated with mitochondria targeted antioxidants?
Plan of investigation
TRPV1 and TRPA1 expression will be measured in a dorsal root ganglion (DRG) cell line treated with paclitaxel, cisplatin, oxaliplatin or Cremophor EL, with and without mitochondria targeted antioxidants for each treatment. Xanthine/xanthine oxidase treatment will be used as an inducer of oxidative stress. Protein Kinase C (PKC) activation will be measured in each treatment condition.
Methodology
Cell surface TRPV1 and TRPA1 expression will be quantified by flow cytometry and fluorescent confocal imaging in DRG cells cultured at a range of concentrations of chemotherapies, or Cremophor EL, with and without mitochondria targeted antioxidants for each treatment. PKC activity will be measured using an enzyme immunoassay kit.
Main outcome measures
Changes in TRPV1 and TRPA1 expression on DRG cells, previously associated with behavioural measures of thermal hyperalgesia.
First year report from Prof H Galley (140 KB)
